TurboScan自動化顯微影像快速掃瞄系統

Mouse

Zebra Fish

Appendix

Mouse Foetus

Eye

Skin

Mouse, Transverse Section

10X Objective / 1X C-mount

Calibration: 0.46 µm/pixel

Scan fields: 17 x 20, 340 total

Size at full resolution: 340 x 4.2 MB = 1.4 GB

Scan area: 10.6 x 9.2 mm = 98 sq. mm

Scan time (snake scan with predictive focus and shading correction): 42 secs

掃瞄後影像全覽

影像放大細節

Zebra Fish

10X Objective / 1X C-mount

Calibration: 0.46 µm/pixel

Scan fields: 16 x 17, 272 total

Size at full resolution: 272 x 4.2 MB = 1.2 GB

Scan area: 9.2 x 7.3 mm = 67 sq. mm

Scan time (snake scan with predictive focus and shading correction): 37 secs

掃瞄後影像全覽

影像放大細節

Appendix, Human

10X Objective / 1X C-mount

Calibration: 0.46 µm/pixel

Scan fields: 16 x 18, 288 total

Size at full resolution: 288 x 4.2 MB = 1.25 GB

Scan area: 10.1 x 8.2 mm = 83 sq. mm

Scan time (raster scan with predictive focus and shading correction): 54 secs

掃瞄後影像全覽

影像放大細節

Mouse Foetus, Lateral Section

10X Objective / 1X C-mount

Calibration: 0.46 µm/pixel

Scan fields: 20 x 16, 320 total

Size at full resolution: 320 x 4.2 MB = 1.3 GB

Scan area: 12.5 x 7.4 mm = 93 sq. mm

Scan time (snake scan with predictive focus and shading correction): 46 secs

掃瞄後影像全覽

影像放大細節

Eye Section, Rabbit

10X Objective / 1X C-mount

Calibration: 0.46 µm/pixel

Scan fields: 19 x 23, 437 total

Size at full resolution: 437 x 4.2 MB = 1.8 GB

Scan area: 11.9 x 10.6 mm = 126 sq. mm

Scan time (raster scan with predictive focus and shading correction): 1 min 19 secs

掃瞄後影像全覽

影像放大細節

Human Skin, Showing Follicles

10X Objective / 1X C-mount

Calibration: 0.46 µm/pixel

Scan fields: 15 x 15, 225 total

Size at full resolution: 225 x 4.2 MB = 0.95 GB

Scan area: 9.4 x 6.9 mm = 65 sq. mm

Scan time (raster scan with predictive focus and shading correction): 38 secs

掃瞄後影像全覽

影像放大細節

Mosaic Acquisition

• Turboscan for fast continuous scan and acquire

• Standard scan for step and acquire for low light applications

• Snake and raster scanning

• User-defined settle time at each field

• Pause and continue scanning mode

• Grab individual fields into mosaic with key-press

• Acquire at full or reduced resolution

• JPEG compression option

• Image streaming for mosaic sizes limited only by free disk space

Specimen Map

• Stage and pattern views

• Point and click relocation to position or pattern field

• Real-time graphic display of current stage position on specimen map

• Zoom and pan

• Drag-drop pattern definition

• Acquire and display multiple mosaics at different magnifications

Scan Patterns

• Rectangle

• Circular

• Annular

• Cross ("+", "x")

• Random

• User-defined

• Save / load scan patterns

• Keyboard support for pattern scanning and stage navigation

Calibration

• X and Y calibration factors

• Stored calibration table for multiple lens and secondary optic settings

• Guided camera to stage alignment procedure

• Automatic correlation-based calibration procedure

• Automatic stage orthogonality correction

Camera Support

• Image setup based on camera capabilities

• Exposure, gain, offset, and gamma control as available

• Automatic and manual white balance

• Color or monochrome acquisition

• Frame-rate shading correction

• Shading correction on live display

• Calibrated graticule overlay on live image

• Grab, copy, save live image

Microscope Automation

• OASIS-4i XY stage and Z focus

• Software joystick for stage, focus, turret, and lamp control

• Leica Microsystems DM range focus, turret, condenser, and lamp control via RS-232

• Olympus BX-61 focus, turret, condenser, and lamp control via RS-232

• Stage and focus speed defined per objective

• Computer controlled parfocality and illumination defined per objective

Export / Import

• Workspace save and load of full mosaic, scan pattern and Surveyor envirnoment

• Export mosaic as full resolution bitmap

• Export mosaic as reduced resolution bitmaps

• Export user-selected region of interest

• Print full mosaic or selected region

• Print preview

Automatic Focus

• Multi-point predictive focus for continuous focus tracking

• Predictive focus setup wizard

• Video autofocus using OASIS-AF module

• Digital camera autofocus using OASIS-DC1 module

• Autofocus parameters stored separately for each objective lens