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INTRODUCTION >> Microscope Eyepiece ReticlesNote: These scales may need to be calibrated, according to intended use. There are a number of uses for the grids and squares listed and they will largely depend on the individual user's application.
Eyepiece Scales
Sectoring: A squared graticule might be used for the systematic examination of a specimen. Some of the squared patterns are numbered to aid in the identification of areas of interest. Sectioning is particularly useful for making drawings of specimens onto graph paper. The chess-board type of pattern helps the user to distinguish the position being examined: the darker squares are translucent, while the lighter ones are transparent, avoiding eyestrain in prolonged counting as may be necessary in hematology. These patterns provide the same advantages when used with image analysis and capture devices.
Counting: A squared graticule can be used for counting. Here, the basic principle is that a small area of the specimen is analyzed in order to obtain information about the total area. This minimizes wasteful work, enabling simple analysis of a particular area. An example of this would be the comparison of large to small particles in a specimen. By using the Miller graticule (NE57) only the smaller particles in the small square are counted, the result being multiplied by ten for comparison with the number of larger particles in the large square.
Squared Grids: Squared grids can be used in particle size analysis as simple technical aids where sophisticated image analysis systems are not required. The areas of the particles to be measured can be estimated by simply counting the number of squares occupied by those particles. It is necessary to estimate fractions of a square or make a rule (e.g. count as a square all partly covered squares at the right and bottom sides of the grid, and ignore partly covered squares at the left and upper sides of the squares). This method would only be useful for a fairly crude estimation of a large diameter.
Squared Grids — NE10, NE11, NE34
Simple grids are convenient for making sketches of the observed specimen on graph paper. They are also useful for particle counting.
Indexed Grids — NE10A, NE11A, NE34A, NE71
Useful for particle counting, particularly when reference is needed between workers. Also, they are useful for area of specimen determinations.
Indexed Grids — NE35
Useful for area of specimen determinations, especially rectangular shapes, also for particle counting.
Chessboard Squares — NE15
The dark squares are translucent. Used as an alternative to simple grids for area of specimen determination and particle counting. Alternative light and dark squares help reduce eyestrain.
Squares — NE38
Combines three areas in one for convenience, giving area ratios A:B of 1:3 and B:C of 1:2.
Miller Squares — NE57
The ratio of large to small squares is 9:1. Originally designed for hematology, they can be utilized for rapid counting of any evenly spread field of particle.
References:
1.American J. of Clin. Pathol. Vol. 20, 1950, page 1079. “Time Saving Device For Counting Reticulocyte.” G.Brescher and Schneiderman.
2.Practicle Haemotology. J.D. Dacy. Published by J.A. Churchill. 2nd Edition 1956 page 25.
Whipple Grid — NE29
Originally designed for water particle analysis, but may be used for other aspects of particle counting. Grid shown: Ratio of full square to smallest is 50:1. Area is 2500:1.
Reference:
Microscopy of Drinking Water.
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Products >> Microscope Eyepiece Reticles
NE10 Net Grid 0.5mm Pitch Squares
Eyepiece Reticle with Grid pattern, 0.5mm pitch, covers whole surface
NE10A Indexed Grid 0.5mm Pitch Squares
Eyepiece Reticle with Grid pattern, 0.5mm pitch, covers whole surface
NE11 Net Grid 1.0mm Pitch Squares
Eyepiece Reticle with Grid pattern, 1.0mm pitch, covers whole surface
NE11A Indexed Grid 1.0mm Pitch Squares
Eyepiece Reticle with Indexed Grid pattern. 10 x 10 grid, 1.0mm pitch squares, marked 1-10 & A-J
NE15 Chessboard 2mm Squares
Eyepiece Reticle with Chessboard pattern, 2.0mm squares, covers whole surface
NE29 Whipple Grid
Whipple Grid 100 squares in 7mm² area (10x10 Grid), centre square further divided into 5 x 5 grid. 10mm² area version also available.
NE29B Whipple Grid
Whipple Grid 100 squares in 10mm² area (10x10 Grid), centre square further divided into 5 x 5 grid. 7mm² standard version also available
NE34 Net Grid 0.1mm Pitch Squares
Eyepiece Reticle with Grid pattern, 0.1mm Squares , covers whole surface
NE34A Indexed Grid 0.1mm Squares
Eyepiece Reticle with Indexed Grid pattern, 10x10 grid, 0.1mm pitch, marked 1-10 & A-J
NE35 Numbered Grid 1mm Square
Eyepiece Reticle with Numbered Grid, 10x10 grid of 1mm Squares, each square numbered from 0 to 99
NE38 Ratio Squares 4, 7, 10mm
Eyepiece Reticle with 3 Squares 10mm, 7mm, 4mm. Area Ratio A:B is 1:3 and B:C is 1:2
NE57 Miller Squares Ratio 9:1
Eyepiece Reticle with Miller Squares pattern, 7x7 mm large square. The ratio of large to small square is 9:1
NE57C Miller Squares Ratio 10:1
Eyepiece Reticle with Miller Squares pattern, 8x8 mm large square with centered smaller square, ratio 10:1
NE71 Indexed Grid 0.5mm Squares
Eyepiece Reticle with 20x20 Indexed Grid pattern, 10mm x 10mm grid with 0.5mm squares, marked 1-20, A-T
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